Lsm 510 meta software

This is the stateoftheart widefield microscope equipped with a co 2incubation system for live cell timelapse experiments. Zeiss 510 meta w zen confocal microscope handbook revised 01152010 startup hbohg lamp on below table little box beside the keyboard. I wrote this a long time ago, so there may be some errors, or differences with different versions of lsm software, but it could be a start. The begin logon windows dialog box appears on the monitor. It is the hbo 50 box sitting on the desk with the monitor 2. Hxp fluorescence illuminator and fluorescent filters for ocular viewing. The lsm 510 and lsm 510 meta laser scanning microscope, including its original accessories and compatible accessories from other manufacturers, may only be used for the purposes and microscopy techniques described in this manual intended use.

The biomolecular research centers zeiss microscope, lsm 510 meta confocal imaging system is located in room 2 of the mathematics building on the campus of boise state university. Zeiss axiovert 200m fluorescencelive cell imaging microscope. Objectives and electronics from carl zeiss guarantee maximum resolution in every application. Lsm 510 meta the difference between seeing a lot and detecting clearly conventional multifluorescence microscopy always reaches its limits when the emission signals of the dyes overlap. Due to four confocal channels for reflected light plus one for transmitted light the lsm 510 is a powerful tool for any application. Zeiss axiovert 200 microscope lsm 510 meta confocal laser scanning microscope laser modul uvir comes with 6 laser lines 405, 458, 488, 514, 543, 633 motorized stage and controller. Due to the large number of import and export filters e. Zeiss lsm 510 meta user manual universitetet i bergen. Zeiss lsm 510 meta laser scanning confocal microscope. Olympus lext ols4000 laser measuring microscope tested w. Attempting to acquire new images in use existing mode will result in a scanned image window with a rainbow pattern, exit program then select new images to correct. Contents starting the zeiss lsm 510 microscope, software. Carl zeiss macro description lsm 510 lsm 510 meta 1. Zeiss lsm 510 meta confocal microscope operating instructions starting the system.

The lsm 510 meta is a very powerful and modular tool that is able to meet your research demands. Inverted options may include a 2 photon ultrafast laser with ndd detection. Finally, the zeiss manual describing the lsm 510 meta confocal system and software for obtaining and analysing images, and the manual which describes the use of the confocor system for fcs use, are both also available for download. Operation lsm 510 software windows nt operating system is being loaded. Turn the black knob on the laser box onequarter turn from off to on. It is hooked to an upright fully motorized axio imager z1 microscope. Alexa 546, bodipy, cy3, dsred, dtomato, rhodamine filter set 49. Zeiss lsm 510 meta inverted confocal microscope m078a smith.

Three lasers capable of providing a total of 7 excitation wavelengths 405 uv range dyes, 458, 477, 488, 514, tunable argon for blue dyes, 543 for red dyes, and 633nm for far red dyes. This microscope has the multichannel meta detector for spectral imaging. Zeiss lsm 510 meta microscope harvard neurodiscovery center. In the operating manual, read the chapter safety instructions carefully before starting operation. We have 1 zeiss lsm 510 meta manual available for free pdf download. Zeiss lsm 510 meta guided tour 7 starting the zeiss lsm 510 microscope, software and laser selecting an objective and focusing the microscope selecting an objective and focusing the microscope configuring the laser scanning and detection for confocal image acquisition acquiring a z and time series data storage. The zeiss lsm 510 microscope has three visible wavelength lasers, a coherent chameleon multiphoton excitation mpe laser tunable from 720nm 930nm a meta spectral emission detector and 4 nlos external detectors. The lsm 510 meta laser scanning confocal microscope from zeiss is a spectral imaging confocal system. The zeiss lsm510 metafcs is situated in the center for cell signaling, 7 th floor, hospital west. Before looking at the sample in the microscope press the vis button. This lsm 510 lsm 510 meta lsm 510 nlo quick guide describes the basic operation of the lsm 510 lsm 510 meta lsm 510 nlo laser scanning microscope with the zen 2009 software. Inverted laser scanning confocal microscope with multiphoton nlo laser this confocal is on an inverted motorized zeiss axiovert 200 microscope. Zeiss lsm image browser is a software program that lets you manage pictures that have been generated with the help of carl zeiss laser scanning system lsm 510 and lsm 5 pascal. Starting the lsm 5 software program lsm 510 double click the lsm 510 icon on the desktop of windows to start the lsm 5 software program.

B16f10 mouse melanoma cells stained with alexa fluor555 and alexa 488 with dna stained with hoechst dye. Flip the two toggle switches on top of the cpu from o to. Zeiss lsm 510 meta inverted confocal microscope m078a smith features include. M i c r o s c o p y f r o m c a r l z e i s s quick guide. Control of the femtosecond laser by the lsm software. Get the best deals on confocal microscope microscopes when you shop the largest online selection at. Turn on the mercury arc lamp if you intend to use it. The system provides high resolution images of fluorescent molecules with improved signal noise ratio compared to wide field fluorescence microscopy. The chameleon provides widely tunable femtosecond pulses with more than 1w of average power. The lsm 510 switchboard window appears on the screen fig. Basic zeiss lsm 510 meta user guide zen software 1. The lsm 510 meta laser scanning confocal microscope from zeiss is a spectral. Click on the scan new images button in the lsm 510 switchboard window. Confocal microscopes zeiss lsm 800 laser scanning confocal microscope az w5 the zeiss lsm 510meta microscope located in az w5 was recently upgraded to an lsm 800.

Files generated by the 510 lsm software can be loaded by the zen. The axiovert observer z1 inverted microscope has filter sets appropriate for dapi, fitc and cy3tritc to view samples in a conventional widefield microscopy mode. The microscope provides capabilities for confocal analysis of cultured cells, tissue sections and other biological specimens. You will need to put down your name on the reservation system. Zen 2009 icon double click on this icon to start the software. Image browser is a free strippeddown version of the lsm 510 software.

Lsm 510 meta confocal microscope zeiss axio observer z1 inverted microscope. Lsm 510 software must be running to use the axiovert 200 microscopelogon to windows with. Zeiss lsm 510 meta user manual hege avsnes dale, molecular imaging center, uib. Zeiss microscope lsm 510 meta confocal with axiovert 200.

A traditional point scanning confocal laser scanning microscope clsm with excitation from 405 to 633 and multichannel imaging. Free lsm image browser comes with many functions from the zeiss lsm software package. Lsm 510 meta confocal microscope molecular, cellular and. Single and batch file export from the lsm 510 in other file formats tif, bmp, avi, quicktime, and more. The lsm 501 meta is set with the buttons for changing the filters positioned just behind the left focus knob, allowing the user to flick between the channels of interest without taking their hands off the microscope controls. Zeiss lsm 510meta confocal laser scanning microscope wur. The purpose of this document is to guide the user to get started with the system as quick as possible in order.

We currently have in stock both inverted and upright lsm 510 confocal systems. Starting the zeiss lsm 510 microscope, software and laser selecting an objective and focusing the microscope configuring the laser scanning and detection for confocal image acquisition acquiring a z and time series data storage descriptions also include the lsm 510 meta. Microscope confocal lsm510 meta welcome to the zeiss lsm 510 meta confocal tutorial. Digital image processing and analysis on a pc running windows 2000 with zeiss lsm 510 meta software package. Lsm 510 upright confocal with meta detector and mp laser isu. The zeiss lsm 510 meta laser scanning confocal microscope in biomedical sciences was purchased in 2004 and has been maintained by zeiss. Copies drawing element of overlay into clipboard and pastes it into other selected windows.

You can minimize it if you like, but do not close it. You will obtain brilliant images with an information content unachievable until now. Lsm 510 upright confocal with meta detector and mp laser. It retrieves all of the image capture and calibration information stored in zeiss. Confocal laser scanning microscopes scan samples sequentially point by point, or multiple points at once. Solveig hehl, tasc carlzeiss jena lsm 510 meta efguided tour april 2002 getting started 1 start the software 3 select laser switch on required laser lines argon laser power should be 60 % 4 select micro choose objective lens from pull down menu select appropriate filterset for fluorescence e. Lsm 510 and lsm 510 meta laser scanning microscopes. Also, the lsm510 software acquires images by scanning a spot of light and reading intensities with a pmt, not a camera.

Clicking on this button activates the complete lsm hardware online mode. Image visart option 0000001207934 3d for lsm option 0000001207919. Manual lsm 510 meta 0 national university of singapore. The objectives currently installed vary from 5x to 63x magnifications. Discover the new lsm 9 family for confocal 4d imaging with high sensitivity and spectral. The zeiss lsm 510 meta has 3 single photon lasers ar, hene 543, hene 633 which allow the user to select lines of 458, 488, 514, 543, 568, and 633 nm. Systempc on components on computer tower on login with your user name and password to biotech domain doubleclick start zen start system initialize hardware e. Under this tab you can turn on and off light to the microscope.

As a result you acquire optical sections with high contrast and high resolution in x, y and z. Zeiss lsm510 axio meta laser scanning confocal microscope system. The lsm 5 lets you implement added lsm software functionality and completely automated problem solutions controlled by macros including everything from lsm setting control to image processing and documentation. This article provides an overview of the zeiss solution of such a combined instrument, the lsm 510 meta confocor 2 system. Carl zeiss macro description lsm 510 lsm 510 meta 1 may 2005 macro solutions. Zeiss lsm 510 confocal inverted microscope system axiovert zoom lsm 510 meta. Lsm meta software is used for imaging and analysis. Guided tour for lsm 510 and meta welcome to marlin. We focus on the instrumental set up as well as technical advances and improvements in the software that controls fcs data acquisition and evaluation. We offer a controller replacement upgrade for the zeiss lsm 510 microscope. Guide to confocal microscopy on the zeiss lsm 510 meta nlo.